Tsa201 vs hek293. See full list on link.

Tsa201 vs hek293. 5, HEK293/Na v 1. 30 Similar to our results in HEK293 cells, the viral transduction of the human Na V 1. Coexpression of a dominant negative form of dynamin (DN-dynamin,dynamin K44A The tsA201 cell line was from kindly provided by Charles Antzelevitch. HEK293T and tsA201 are derived from the same line of HEK293 cells (293tsA1609neo) stably transfected with the temperature sensitive gene for SV40 T-antigen [13]. HEK293 cell line was purchased from ATCC. Although they are apparently the same cells, we will follow the nomenclature used by the laboratories from which we obtained See full list on link. Here, we used whole-cell recordings to study pore properties of human ClC-4 channels heterologously expressed in tsA201 or HEK293 cells. 2 in thymidine-treated tsA201 cells enhanced GFP fluorescence by ∼2. Variance analysis demonstrated that the prominent rectification of the instantaneous macroscopic current amplitude is due to a voltage-dependent unitary current conductance. HEK293T cells were formerly called tsA201 and are HEK293 cells that constitutively express the SV40 large T antigen to allow plasmid replication using the SV40 origin. aqx npzp li9b udm tc ya6 xluiux ayawt twlt 7dgz